Last Updated: 2/2025
Summary: This article covers potential factors and solutions to consider before restarting MLF.
There are many factors that can influence the success of a malolactic fermentation (MLF). Before jumping into a full restart, it is a good idea to assess each of these factors. Sometimes only a small adjustment is needed to help MLF complete successfully. Use the guide below to evaluate the potential causes and solutions.
STEP 1: DETERMINE IF WINE CONDITIONS ARE ANTAGONISTIC TO BACTERIA
Malolactic bacteria are affected by wine conditions and bacteria should be chosen with this in mind. However, if MLF becomes stuck or sluggish, the first step is to evaluate whether wine conditions are problematic:
TEMPERATURE MIGHT BE TOO LOW
Low temperature is the most common reason for slow and stuck MLF. Cellar temperatures are often significantly lower than the optimal range for ML bacteria. Try warming the tank or barrels to 18-20°C (64-68°F).
ALCOHOL MAY BE TOO HIGH
If the alcohol level is higher than the tolerance of the bacteria, a restart may be necessary (using a strain with a higher alcohol tolerance).
pH MAY BE TOO LOW
If the wine pH is lower than the tolerance of the bacteria, a restart will be necessary using a pH-compatible strain.
SO2 MAY BE TOO HIGH
Even if little or no SO2 has been added, it may still be present during MLF. SO2 can come from several sources including yeast, old barrels, and/or erroneous cellar additions.Â
If total SO2 (TSO2) has exceeded the tolerance of the bacteria strain, the wine should be reinoculated with a strain that has a higher TSO2 tolerance. TSO2 may also be lowered via blending.
MALIC AND LACTIC ACID MAY BE TOO HIGH
Lactic acid >1.5 g/L can slow MLF and levels >3 g/L can inhibit MLF. If initial malic acid >7.0 g/L, an inhibitory amount of lactic acid may be produced from the malolactic conversion and a complete MLF may not be possible without blending or other corrective actions.
STEP 2: IF WINE CONDITIONS ARE NOT ANTAGONISTIC (OR HAVE BEEN ADJUSTED), CONSIDER THE FOLLOWING:
THE BACTERIA MIGHT BE STRUGGLING TO STAY IN SUSPENSION
If the wine has low turbidity, bacteria may struggle to stay in suspension. Try stirring tanks or barrels more frequently.
THE WINE MIGHT BE LACKING NUTRIENTS
Malolactic bacteria have specific nutrient needs, but there are no easy/cost-effective analyses that can help determine deficiencies in wine. ML bacteria require organic acids (malic, citric, pyruvic), organic nitrogen (amino acids, peptides), vitamins (B group), trace minerals (Mn, Mg, K, Na), and low levels of sugar (fructose, glucose). If any nutrients are deficient, bacteria may not consume malic acid.Â
Malolactic nutrients can compensate for deficiencies. As it is difficult to determine whether a wine is deficient, we recommend using them preventatively or intervening with a nutrient add within 7 days of inoculation if MLF has not started (and temperature is not problematic). Adding a malolactic nutrient is not as critical if bacteria is co-inoculated with yeast.
THE WINE MIGHT NEED A DETOX
If you have already added ML nutrients, the bacteria should have what it needs. Sometimes toxins can be present that inhibit MLF. RESKUEâ„¢, a specific inactivated yeast for treating stuck fermentations, can be extremely beneficial for detoxification. 30 g/hL RESKUE should be added and racked after 48 hours.
THERE MAY NOT BE ENOUGH BACTERIA
If you did not inoculate with Oenococcus oeni, there may not be enough healthy bacteria. In order for MLF to begin, there must be 1 million cells per mL (and native strains may not be able to achieve this population). Consider inoculating with a commercial strain.
IT MIGHT JUST NEED MORE TIME
MLF can be a test of patience taking weeks, or even months, to complete. To determine if MLF is complete, malic acid must be measured — it is nearly impossible to determine completion by sensory analysis — and is considered complete when malic acid is ≤0.1g/L (some say ≤0.2 g/L).
STEP 3: RESTART
If the sluggish malolactic fermentation cannot be fixed by changing wine conditions or by trying any of the other solutions presented, click here for restart instructions.
READ PROTOCOLLearn More
To avoid stuck and sluggish malolactic fermentations, consider using the Malolactic Fermentation Feasibility Evaluation. The scorecard rates how antagonistic several wine conditions are to ML bacteria and returns a total point value representing the predicted difficulty of MLF. Based on this value, Scott Laboratories recommends certain steps be taken to maximize success.
READ PROTOCOLLast Updated: 2/2025
Summary: This article covers potential factors and solutions to consider before restarting MLF.
There are many factors that can influence the success of a malolactic fermentation (MLF). Before jumping into a full restart, it is a good idea to assess each of these factors. Sometimes only a small adjustment is needed to help MLF complete successfully. Use the guide below to evaluate the potential causes and solutions.
STEP 1: DETERMINE IF WINE CONDITIONS ARE ANTAGONISTIC TO BACTERIA
Malolactic bacteria are affected by wine conditions and bacteria should be chosen with this in mind. However, if MLF becomes stuck or sluggish, the first step is to evaluate whether wine conditions are problematic:
TEMPERATURE MIGHT BE TOO LOW
Low temperature is the most common reason for slow and stuck MLF. Cellar temperatures are often significantly lower than the optimal range for ML bacteria. Try warming the tank or barrels to 18-20°C (64-68°F).
ALCOHOL MAY BE TOO HIGH
If the alcohol level is higher than the tolerance of the bacteria, a restart may be necessary (using a strain with a higher alcohol tolerance).
pH MAY BE TOO LOW
If the wine pH is lower than the tolerance of the bacteria, a restart will be necessary using a pH-compatible strain.
SO2 MAY BE TOO HIGH
Even if little or no SO2 has been added, it may still be present during MLF. SO2 can come from several sources including yeast, old barrels, and/or erroneous cellar additions.Â
If total SO2 (TSO2) has exceeded the tolerance of the bacteria strain, the wine should be reinoculated with a strain that has a higher TSO2 tolerance. TSO2 may also be lowered via blending.
MALIC AND LACTIC ACID MAY BE TOO HIGH
Lactic acid >1.5 g/L can slow MLF and levels >3 g/L can inhibit MLF. If initial malic acid >7.0 g/L, an inhibitory amount of lactic acid may be produced from the malolactic conversion and a complete MLF may not be possible without blending or other corrective actions.
STEP 2: IF WINE CONDITIONS ARE NOT ANTAGONISTIC (OR HAVE BEEN ADJUSTED), CONSIDER THE FOLLOWING:
THE BACTERIA MIGHT BE STRUGGLING TO STAY IN SUSPENSION
If the wine has low turbidity, bacteria may struggle to stay in suspension. Try stirring tanks or barrels more frequently.
THE WINE MIGHT BE LACKING NUTRIENTS
Malolactic bacteria have specific nutrient needs, but there are no easy/cost-effective analyses that can help determine deficiencies in wine. ML bacteria require organic acids (malic, citric, pyruvic), organic nitrogen (amino acids, peptides), vitamins (B group), trace minerals (Mn, Mg, K, Na), and low levels of sugar (fructose, glucose). If any nutrients are deficient, bacteria may not consume malic acid.Â
Malolactic nutrients can compensate for deficiencies. As it is difficult to determine whether a wine is deficient, we recommend using them preventatively or intervening with a nutrient add within 7 days of inoculation if MLF has not started (and temperature is not problematic). Adding a malolactic nutrient is not as critical if bacteria is co-inoculated with yeast.
THE WINE MIGHT NEED A DETOX
If you have already added ML nutrients, the bacteria should have what it needs. Sometimes toxins can be present that inhibit MLF. RESKUEâ„¢, a specific inactivated yeast for treating stuck fermentations, can be extremely beneficial for detoxification. 30 g/hL RESKUE should be added and racked after 48 hours.
THERE MAY NOT BE ENOUGH BACTERIA
If you did not inoculate with Oenococcus oeni, there may not be enough healthy bacteria. In order for MLF to begin, there must be 1 million cells per mL (and native strains may not be able to achieve this population). Consider inoculating with a commercial strain.
IT MIGHT JUST NEED MORE TIME
MLF can be a test of patience taking weeks, or even months, to complete. To determine if MLF is complete, malic acid must be measured — it is nearly impossible to determine completion by sensory analysis — and is considered complete when malic acid is ≤0.1g/L (some say ≤0.2 g/L).
STEP 3: RESTART
If the sluggish malolactic fermentation cannot be fixed by changing wine conditions or by trying any of the other solutions presented, click here for restart instructions.
READ PROTOCOLLearn More
To avoid stuck and sluggish malolactic fermentations, consider using the Malolactic Fermentation Feasibility Evaluation. The scorecard rates how antagonistic several wine conditions are to ML bacteria and returns a total point value representing the predicted difficulty of MLF. Based on this value, Scott Laboratories recommends certain steps be taken to maximize success.
READ PROTOCOL