Non-Saccharomyces Yeast Rehydration

Last updated: 6/2021

Applies to: Winemakers rehydrating non-Saccharomyces yeasts. This protocol is NOT applicable to Saccharomyces yeast rehydration. For more information on Non-Sacc yeasts and advice on selection, see our article "Harnessing the Unique Powers of Non-Saccharomyces Yeasts."


While they require a bit of extra care, the non-Saccharomyces yeasts in our portfolio are powerful tools to enhance wine quality and allow the winemaker to achieve their desired wine style. Be sure to keep in mind the following when working with Non-Sacc yeasts:

Secondary inoculation:

A secondary inoculation with Saccharomyces is necessary to ensure a complete alcoholic fermentation. This is because non-Sacc yeast have a very low tolerance to ethanol and CO2, therefore their activity and viability drops once alcohol reaches approximately 5% v/v. YAN will need to be adjusted accordingly for the secondary Saccharomyces inoculation.

Strain-specific instructions:

Consult strain-specific instructions when using a non-Saccharomyces yeast for two reasons:

  • Non-Sacc yeasts need to be rehydrated differently from Saccharomyces yeasts.
  • The timing of your secondary Saccharomyces inoculation will depend on the Non-Saccharomyces strain you have selected.

Starting conditions:

Before beginning, ensure the starting conditions of your wine are compatible with the selected Non-Sacc yeast strain:


FSO2 (ppm)



<50 TSO2

YAN (ppm)






  • step 1: Rehydrate your non-Sacc yeast in 10x its weight of chlorine-free water at 30°C (86°F). Stir.
  • step 2: Wait 15 minutes and stir again.
  • step 3: Slowly add must to the yeast slurry until the temperature of the yeast slurry drops by 10°C. Wait 15 min. Repeat this step until slurry is within 10°C of must. NOTE: this step should not exceed 45 minutes total.
  • step 4: Inoculate

After inoculating with your chosen Non-Sacc yeast strain, consult the chart below to determine how long to wait before completing a Saccharomyces inoculation. Click here for Saccharomyces yeast rehydration instructions.


When to add Saccharomyces:

After 1.5-3 °Brix drop

24 hours after FLAVIA

Upon juice receipt or after cold soak
Once juice is racked to fermentation vessel (white/rosé)
24-72 hours after LAKTIA