Rehydration Phase

• Mix and dissolve the contents of the activator in the appropriate amount of 20 ºC (68 ºF) water (see below).
• Add the contents of the bacteria sachet and dissolve carefully by gentle stirring.
• Wait for 20 minutes.

Acclimatization Phase

• Add the bacteria/water mixture from the previous step with an equivalent volume of wine. The pH of the wine should be over 3.5 and temperature between 18-25 ºC (64-77 ºF).
• Wait for 18 to 24 hours.

Pre-Culture Transfer Phase

• Transfer the pre-culture to the balance of wine in the tank.
• Maintain the temperature between 18-25 ºC (64-77 ºF).
• Check MLF activity (malic acid degradation) every 2 to 4 days.