Lallemand 1-Step Build-Up Cultures

Instructions for Rehydration

Rehydration Phase

  • Mix and dissolve the contents of the activator in the appropriate amount of 20 ºC (68 ºF) water (see below).
  • Add the contents of the bacteria sachet and dissolve carefully by gentle stirring.
  • Wait for 20 minutes.

Acclimatization Phase

  • Add the bacteria/water mixture from the previous step with an equivalent volume of wine. The pH of the wine should be over 3.5 and temperature between 18-25 ºC (64-77 ºF).
  • Wait for 18 to 24 hours.

Pre-Culture Transfer Phase

  • Transfer the pre-culture to the balance of wine in the tank.
  • Maintain the temperature between 18-25 ºC (64-77 ºF).
  • Check MLF activity (malic acid degradation) every 2 to 4 days.
Bacteria Dosage SizeWater/Wine Needed for Build-UpTransfer Culture Into
25 hL0.65 gallons650 gallons
100 hL2.6 gallons2,600 gallons
500 hL13 gallons13,000 gallons
1,000 hL26 gallons26,000 gallons
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